A

Annexin

nnexin VV

检检测细胞凋亡

测细胞凋亡 ........................................................................................2

实验原理................................................................................................................2

实验用品................................................................................................................2

操作步骤................................................................................................................3

Annexin V Blocking ..............................................................................................5

凋

凋亡细胞的

亡细胞的 DDNA

NA 断断裂片段分析

裂片段分析 .............................................................................7

实验原理................................................................................................................7

实验用品................................................................................................................8

操作步骤................................................................................................................9

B

BrdU

rdU FFlow

low KKits

its 检检测细胞增殖

测细胞增殖..............................................................................12

实验原理..............................................................................................................12

BrdU Flow Kits 试剂盒.......................................................................................12

结果分析..............................................................................................................17

流式仪器设置指南..............................................................................................18

线

线粒体膜电位变化检测细胞凋亡

粒体膜电位变化检测细胞凋亡..........................................................................22

实验原理..............................................................................................................22

实验用品..............................................................................................................22

样本制备..............................................................................................................23

结果分析..............................................................................................................24

注意事项..............................................................................................................24

A

Active

ctive CCaspase-3

aspase-3 检检测细胞凋亡

测细胞凋亡 ...........................................................................26

实验原理..............................................................................................................26

实验步骤..............................................................................................................27

结果分析..............................................................................................................28